36 Replies to “Green Fluorescent Protein | What is this Thing?!”

  1. Cooool… if you need to make me glow as part of your PhD jazz I might be okay with donating my (living) body to Science! 😀

  2. If you liked what you just saw, check out "brainbow" by Lichtman and Sanes. they used a variety of different fluorescent proteins to mark individual neurons, which is not only fascinating but also beautiful

  3. Wow, I really don't know enough about biology to understand the stuff explained in this video. Why is GFP only glowing when a gene is active? How can it be attached to one single gene, and not the whole DNA, I mean this is microscopical! All these basics are what I'm missing to make sense of this video. But I liked it anyways, because I like watching Alex explain science stuff 😉 (I only wish she were doing physics – sooo much more interesting, LOL!) ;-))

  4. Really impressive, no wonder this won the 2008 Noble prize in chemistry!

    I was hoping you had shown the fluorescence of that E.coli broth in action using some kind of lamp!

    Do you use this technique in conjuction with like confocal microsopy to get information on where the genes are expressed?

  5. At 1:00 you say you could use gfp on a gene that has a very clear phenotype. Did you mean to say one that has a very unclear phenotype?

  6. Bacterial Paintings.. now there's a term I didn't think I'd ever hear or want to know more about, but here we are! 😛

  7. I'm a huuuuggggeeee fan of your channel. Thanks teach us about cool science stuff in a way that is very easy to understand. Very cool! Keep rockin' it!

  8. Why do scientist always have e. coli samples in their labs? Isn't that the bacteria that causes food poisoning? Wouldn't the regular use of this bacteria cause some mutations, making it harder to work with?

    Thanks 🙂

  9. Great video! There's an interesting side story about GFP. Doug Prasher is often regarded as having come up with the concept for GFP's modern uses, but his grant to develop it was turned down by the NIH. He ended up leaving academia due to his inability to gain funding, only to have his work on GFP turn into the Nobel Prize winning scientific tool it became (through the work of his lab mates, including Chalfie).

    It is a fascinating and terrifying story about the vagaries of grants and academia.

  10. Ordinary human beings use mere highlighters and pens . . . Super Hero Biologists use the very building-blocks of life itself to mark their works with.

  11. Hello, I was wondering if I could have your email (not to be creepy) to discuss more on Green Fluorescent Proteins as my friend and I are trying to conduct a science fair project on this particular topic, but it is not in our current class curriculum and you seem to know A LOT about it. I would really like to talk in depth with you because, frankly, me researching (or trying to research) academic articles necessary for the science fair isn't making doing it nor am I learning anything new. Thank you!

  12. Amused by your lab coat, protecting your chair behind you! In my institute, not wearing PPE would be a disciplinary. Probably need to think about whether you need to send a better message? (p.s. it may sound like sour grapes, but there have been too many serious incidents no to take this seriously)

  13. GFP discovered first by Douglas Prasher, who ended up driving a bus while the other guys one the Nobel Prize.

  14. Hi Alex, Thanks for the great video. Can you please explain or point to something that explains, regarding how do you get the E.coli bacteria to synthesise green fluorescent protein in the first place. What do you have to add to e coli to do so ?
    Many Thanks !

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