Jess: Your Complete Protein Analysis Solution For Automated Western Blots

Jess: Your Complete Protein Analysis Solution For Automated Western Blots


Protein analysis comes with many
challenges, labor intensive protocols, increase time to result, and multiple
hands-on steps increase user error and data variability. Meet Jess your protein
analysis problem solver. Jess automates the protein separation and immunodetection of traditional Western blotting eliminating many of the tedious
error-prone steps. Just load your samples and reagents into the micro plate, insert
the plate and capillary cartridge and Jess does the rest. She separates your
protein by size and precisely manages antibody additions, incubations, washes,
and even the detection steps. Come back to fully analyzed and quantitated
results in three hours. Jess, she’s like Western blot meets ELISA in one. Jess
gives you four ways to analyze proteins. her fluorescent detection enables
multiplexing, letting you maximize your time and sample. With Jess’
chemiluminescent detection you’ll get picogram level sensitivity letting you
maximize the data you get from your sample. Her in capillary protein
normalization reagent is an easy way to see if your samples contain a consistent
protein load and investigate experimental setup and user errors.
You’ll be able to publish your results with confidence with effective
normalization of your target protein expression and if you’re still doing
traditional westerns – snap get the picture with Jess’s inbuilt plot imaging
system. Simple Western immunoassays take place in a capillary your sample,
separation matrix, stacking matrix, antibodies, and reagents are loaded
automatically from a specially designed plate. Jess begins by aspirating the
separation matrix and then the stacking matrix into each capillary. Next your protein lysate is loaded and
capillaries are lowered to make contact with running buffer. Voltage is applied
to enable separation by molecular weight once the separation is complete, UV light
immobilizes the proteins to the capillary wall. With proteins now
immobilized and the matrix cleared of the capillary Jess starts the amino
probing process. For chemiluminescent detection samples are first incubated
with the primary antibody, followed by a secondary HRP conjugate, and finally
chemiluminescent substrate. The chemiluminescent reaction is recorded by
a CCD camera in a series of images over time. For fluorescent detection, samples
are incubated with the primary antibody followed by infrared or near-infrared
fluorescent secondary tagged antibodies. Excitation of the fluorophores releases
photons and the emission spectra is detected and recorded by a CCD camera in
a series of images over time. Protein normalization has never been easier with
Jess. Just load her protein normalization reagent into a row of
wells on the plate and she’ll take care of the rest.
The fluorescent labeled reagent reacts with a means on proteins enabling a
between capillary comparison of protein load. Best of all Jess’s florescent
detection capabilities enable to color protein detection for multiplexing on
top of protein normalization. Analysis is a breeze want to identify whether a
protein is present or absent? Jess gives you the qualitative Western blot data
you are used to seeing. Even better she’ll quantitate the data
for you too. With a few clicks you’ll be analyzing immunoassay like
standard curves and precisely quantifying your protein. With protein
normalization you can take your protein load comparison and transform your data
to effectively normalize your samples increasing your confidence in your data
interpretation. Jess, she’s like Western blot meets ELISA in one.

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